Wondering when to do pregnancy test after IUI? At primeivfcentre, we recommend waiting at least 14 days post-IUI before taking a pregnancy test. Testing too early can result in inaccurate outcomes due to residual hormones from fertility medications. The two-week wait allows enough time for implantation and the production of detectable hCG levels. If you experience early signs like mild cramping or breast tenderness, resist testing too soon.